ZEISS Knowledge Base

Flow View

This view displays your experiment and the flow information. The different areas containing the image, the charts and the table can be resized by dragging the individual area boundaries with the pressed left mouse button.

1	Preview Image Here you can see the acquired preview image with the measurement positions marked in the view.
2	Parameter Table This table displays the data for the different measurement positions. Each position is displayed as a row. For details, see Flow Parameter Table.
3	Flow Diagram This diagram illustrates the flow based on the Airyscan detector. The arrow indicates the flow direction. For information about the flow calculation and the classification for flow speed, see Flow Calculation. The displayed degrees can vary based on your system configuration. A right click into the area enables you to export the flow diagram as an image.
4	Correlation Chart Here you have the correlation chart for the individual spots. You can zoom into the displayed curves with the mouse wheel or by drawing a zoom rectangle with pressed left mouse button. A right click in the chart area resets the chart (zoom) to the default view.
5	View Options Here you have the area for your standard view options as well as the specific options provided on the Flow Tools tab, see Flow Tools Tab.

Flow Parameter Table

This table displays the data for the different measurement positions. Each position is displayed as a separate row. If a value could not be calculated for a specific position, the corresponding entry in the table is empty. A click on an entry highlights and selects the entire row and displays the corresponding data in the chart. The units for individual parameters are displayed as tooltips. A right click in the table opens a context specific menu, see Parameter Table Context Menu.

Parameter	Description
Ø	Activated: Uses this position for the calculation of an average for individual values. The average is displayed on the bottom of the table.
Visibility	Toggles the visibility of the respective spot.
ID	Displays the ID for the individual spot.
Color	Sets the color for the respective measurement point by clicking on the field and selecting a color form the dialog.
Name	Displays and sets the name for the individual position.
Count Rate	Displays the count rate in kHz.
CPM	Displays the CPM in kHz. The color of the value indicates the data quality, see Data Quality Criteria (CPM).
Data Quality	Displays an indication for the fit quality.
Number of Molecules	Displays the number of molecules found at the measurement position. It is calculated by 1/correlation amplitude, where the correlation amplitude is defined as value of the correlation curve at the smallest lag time.
Flow Direction	Displays the value that indicates the flow direction.
Flow Speed	Displays the flow speed in µm/s.
Flow Speed Confidence Interval	Displays the confidence interval for the flow speed.
Fit Model	Displays which fit model was used.
Date	Displays the date and time of the experiment.

Flow Tools Tab

Parameter		Description
Reference Image		Only visible if you have created a second reference image after the spot experiment. Selects which reference image is displayed int the view.
–	Pre-Experiment	Displays the reference image acquired before the experiment.
–	Post-Experiment	Displays the reference image acquired after the experiment.
Correlation Chart		Selects which curves are displayed in the correlation chart and allows you to set the color for the curves.
Fit Model		Selects the fit model for the correlation curve.
–	1 Component 2D	Selects the one component 2D model.
–	1 Component 3D	Selects the one component 3D model.
–	Show Fitted Curves	Activated: Displays the fitted curves in the chart.

Flow View

Flow Parameter Table

Flow Tools Tab

See also

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