Adjust Airyscan Detector

Adjustment

Starts an automatic detector adjustment. When the adjustment is running, it changes to a Stop button.

Quality and Status

Displays the status of the Airyscan detector adjustment.

Detector View

Displays the current intensity distribution of the emission signal over the detector elements.

The following parameters are only available if the automatic adjustment fails:

Zoom

Sets the zoom level. Clicking the 1.x button behind the input field resets the zoom level to the default.

High Intensity Laser Range

Activated: Enables the higher laser power range between 0.2 and 100%. This setting is applied to all laser lines.

Laser

Set the required attenuation (%) for the laser.

Master Gain

Sets the master gain to control the voltage of the PMTs. Increasing the gain of the PMT corresponds to a higher voltage of the detector. The image gets brighter and you may be able to reduce the laser power.
With a higher voltage, the noise level in the image increases as the dark noise of the detector gets visible in the images, predominantly as single bright pixels. The optimum between gain and noise depends on your experimental requirements and on your sample.

Scan Speed

Sets the scan speed with the slider or input field. The corresponding values for Frame Time and Pixel Time are displayed above the slider.

Fiber Position

Displays controls for the manual adjustment of the fiber positions.

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X Position

Changes the adjustment of the fiber in x.

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Y Position

Changes the adjustment of the fiber in y.

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Store Invis Correction Position Automatically

Activated: Saves and stores the current values for the next alignment.

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Store Current Pos.

Stores the current positions of x and y for further reference.

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Move To Stored Pos.

Moves to the currently stored position and updates the X Position and Y Position accordingly.