The settings of Auto Z Brightness Correction are part of the image acquisition and are reused with other settings of an image. They are also part of an experiment setting. However, the function is not activated for reuse or when loading an experiment as the settings apply to the absolute z-position in µm used when the (previous) image was acquired. If the new stack is acquired in a different position, using the previously defined settings (extrapolate) can lead to a an extreme overexposure of the sample. Enable test is always deactivated when an image stack is reused, or an experiment is loaded.

Worflow:
In order to reuse the Auto Z Brightness parameters for subsequent z-stacks, make sure to manually set the center (or first or last) position of the first z-stack to zero (Focus TW, Z-position: Set Zero) before defining the Auto Z parameters. Set the center (or first or last) position of all following Z-Stacks also to zero to be able to reuse the Auto Z Brightness parameters accordingly. When saving and loading the parameters, the same logic applies. When switching between linear and spline interpolation during continuos scan, the current acquisition parameters are not updated until the z-position is changed.