Airyscan Processing Parameters

3D Processing

Only available for images with 5 or more z-positions.
If activated, this option improves the resolution in axial and lateral direction. The data set needs to have at least 5 z-sections acquired with an optimal step size. 3D Processing is slower than 2D Processing. For 3D Processing, the whole z-stack (single channel and time point) needs to fit into the physical memory.

2D SR Processing

Only available for 2D images.
Enhances the 2D resolution. Note that this only results in increased super resolution when images are acquired with optimal settings and sufficient signal.

Auto Filter

If activated, a suitable Super Resolution parameter for the Airyscan processing is automatically determined for the selected data set. To manually adjust the Super Resolution parameter, deactivate the checkbox.

Strength

Use this option for an increased (high) or decreased (low) strength of the automatically assigned filter value. This is especially useful for 3D processing, as the 2D preview of the processing filter value in the Airyscan viewer does not allow to conclude the result after a 3D data processing.
The increment of this parameter is ± 0.4 compared to the standard auto Airyscan processing. This setting is not available when manual processing strength is selected.

Adjust per Channel

Only visible if Auto Filter is deactivated and only for images with two or more Airyscan channels.
If activated, you can manually set channel specific Airyscan processing parameters.

Super Resolution

Only available if Auto Filter is deactivated.

Manually adjusts the Super Resolution parameter.
Note: High strength might look attractive at some images, Z planes or color channels, but other filtering artefacts might occur which appear like small rings in the image. Also the results will become very sharp, but grainy. So carefully check your image data in order to avoid such artefacts.