1. If you are using Direct Processing on different computers, you have connected acquisition and processing computer, see Connecting Acquisition Computer and Processing Computer.
  2. To ensure that the processing computer reads incoming files and starts the processing, on the Applications tab, in the Direct Processing tool, you have clicked Start Receiving. This is usually active by default.
  3. On the Acquisition tab, Direct Processing is activated. This activates the Auto Save tool as well.
  4. Depending on your settings, you have defined the folder where the acquired images are stored in the Direct Processing or the Auto Save tool. Use a folder to which the processing computer has access. For information about sharing a folder, see Sharing a Folder for Direct Processing.
  5. On the Acquisition tab, you have set up your experiment for image acquisition.
  6. If you want to use advanced settings created with the Deconvolution (adjustable) function, you have the settings available, see also Creating Deconvolution Settings.
  1. On the Acquisition tab, open the Direct Processing tool.
  2. If no Direct Processing settings were made before for the current experiment, a particular processing function is already preselected depending on your microscope, channel settings and licenses.
  3. From the Processing Function dropdown list, select Deconvolution.
  4. Select a deconvolution method. We recommend Excellent, slow (Constraint Iterative). If you want to use an advanced settings file you have created with the function Deconvolution (adjustable), activate Use Advanced Settings.
  5. A dropdown list is displayed under Load Setting created in the Deconvolution function.
  6. In the drop-down list, select your advanced settings for Deconvolution.
    Note: Currently Direct Processing supports settings configured in the Deconvolution tab of the image processing function Deconvolution (adjustable) and some parameters of the PSF Settings tab. Especially parameters that rely on external data (like using external PSF) are not possible with Direct Processing.
  7. Set up the experiment. For optimal processing efficiency, select the Full Z-Stack per channel option. This way, the processing can start as soon as a channel-z-stack has been completed. Keep in mind that for Colocalization studies better results might be achieved when the default All Channels per Slice is used, depending specific application and filter configuration.
  8. Click Start Experiment to run the experiment. Note: You can pause the processing. If you stop the experiment, requests that have been sent earlier by the acquisition computer are not processed. However, already processed images will be retained.
  9. The images are stored in the folder you have defined in the Auto Save or Direct Processing tool. When you abort the acquisition, the remote processing will not take place. In case you have set up several processing functions, only the acquired image and the final output image are stored.
  10. The processing computer reads incoming files and starts the processing. The path to the selected folder, the currently processed image as well as the images to be processed are displayed in the Direct Processing tool. The processed image is saved to the same folder specified in the Direct Processing tool. If the image name already exists in this folder, the new file is saved under a new name <oldName>-02.czi.
  11. To cancel the processing on the processing computer, on the Applications tab, in the Direct Processing tool, click Cancel Processing.
  1. Once processing is finished, you are notified on the acquisition PC and can open and view the acquired image as well as the processed image. This should be done on the processing computer, so that you can immediately start a new experiment on the acquisition computer. However, you can also automatically open the processed image on the acquisition PC with the respective setting in the Direct Processing tool on the Acquisition tab.
  2. When you open the image, in the Image View, on the Info view tab, information about the executed deconvolution is available. When Deconvolution is done through Direct Processing, the info about Deconvolution parameters shows the suffix online and the Convergence History graph. Additionally, general information about Direct Processing (e.g. the duration) is also available on the Info view tab of the processed image.