Note that the z values of positions, tile regions (unless they have at least one support point), local support points, and global support points are verified in a separate dialog accessed via the Focus Surface and Support Points section. As the dialog contains the same items and options for verifying the Z values it is described here once.
|
Parameter |
Description |
|
|---|---|---|
|
Tile Regions/Positions List |
Displays all the tile regions (TR), positions (P), or support points (SP) currently active in your experiment. The list contains the following columns and buttons: |
|
|
- |
Status |
Here you can see if the Z-Position is already verified. Then a green checkmark will appear in the corresponding row. |
|
- |
Name |
Displays the name of the selected object. |
|
- |
Z (µm) |
Displays the z-position of the tile region, position, or support point. |
|
- |
Tile Region |
Shows the tile region to which the local support point belongs. |
|
- |
Array |
Shows if the position is part of a Position Array. |
|
- |
|
Opens the options menu for verifying tile regions/positions, see description below. |
|
Verification Helper Method |
||
|
- |
None |
If selected, you need to manually adjust the focus for each point to be verified. |
|
- |
Autofocus (AF) |
Only available if your system has a motorized focus drive (z-axis) and the software autofocus module. If selected, you can use the software autofocus for adjusting the focus for each point to be verified. The corresponding buttons will then appear in the dialog. |
|
- |
Reflex Autofocus (AF) |
Only available for Lattice Lightsheet. |
|
- |
Definite Focus (AF) |
Only available if your system has a Definite Focus device. If selected, you can use the definite focus for adjusting the focus for each point to be verified. The corresponding buttons will then appear in the dialog further below. |
|
Move to Current Point |
Moves the stage to the selected object. |
|
|
Include Z when Moving to Points |
Activated: ZEN moves to the selected object and adjusts the position of the z-drive to the currently assigned z-value. |
|
|
Set Z & Move to Next |
Sets the current z value for the selected support point and sets the status to verified. Then the software moves the stage to the next support point. |
|
|
Run AF (or DF) and Set Z |
This button is only visible if you have selected Autofocus (AF), Reflex Autofocus (AF), or Definite Focus (DF) as Helper Method. Runs the software autofocus/definite focus and sets the current z position to verified. |
|
|
Use AF (or DF) to Verify the Remaining |
This button is only visible if you have selected Autofocus (AF), Reflex Autofocus (AF), or Definite Focus (DF) as Helper Method. Automatically moves to the remaining points and determines the z value using the selected autofocus option for each point. |
|
Options for verifying tile regions/positions
|
Parameter |
Description |
|---|---|
|
Current Point Verified |
Here you can change the status of the selected support point from verified to unverified (or vice versa). |
|
Set all Points as Verified |
Changes the status of all points to verified. |
|
Reset Verification State of all Points |
Changes the status of all points to unverified. |
|
Set Current Z for Selected Points |
The current z value is set for all selected points. You can press Ctrl and the left mouse button to select multiple points, or Ctrl + A to select all. |
|
Set Current Z for all Points |
The current z value is set for all points. |
|
Apply Z-Offset... |
Opens al dialog to apply a z-offset for all or the selected points. |