NOTICE

notice

Risk of camera mismatch on dual-camera systems

Changing the scaling on a dual-camera system can cause a mismatch between the two cameras, which results in a shift between the images of the individual cameras.

  1. Scaling should not be changed for dual-camera systems of the Lattice SIM family (Lattice SIM 3, Lattice SIM 5, ELYRA 7 with Lattice SIM) as this will affect camera synchronization. Contact your local Service Support if the scaling was changed.

Availability of created scaling

If you create a manual scaling, this data is by default user specific, i.e. the scaling is not globally available for all users of the PC. The scaling is saved in C:\Users\Username\Documents\Carl Zeiss\ZEN\Documents\Scalings.

  1. If you create a scaling that should be available to all users on the PC, you have to place the file into C:\Program Files\Carl Zeiss\ZEN 2\ZEN 2 (blue edition)\ZEN\en\Documents\Scalings.
  1. You have an object micrometer oriented horizontally on the microscope stage.
  2. You have defined all your microscope settings correctly with the Microscope Control tool, or the Microscope Components tool (ZEN lite only). In our example we use an objective with a 10x magnification.
  1. Acquire an image of the scale in your object micrometer using the objective to be scaled manually, see also Acquiring Camera Image.
  2. The image is displayed in ZEN.
  3. In the bottom status bar click the small arrow in the Scaling section.
  4. The Scaling dialog opens.
  5. In the dialog, deactivate Select Automatically.
  6. In the Create new scaling section, click Interactive Calibration....
  7. The calibration window opens in the image area.
  8. Click Single Reference Line (selected by default) and activate Automatic Line Detection.
  9. Draw in the reference line along the scale.
  10. Enter the true distance between both scale lines in the calibration window. In our example this is 500 micrometers.
  11. Enter a name for the scaling (i.e. Obj 10x) and click Save Scaling.
  1. You performed a manual scaling for your objective. Repeat this sequence for all objectives you need a manual scaling for. Always ensure that you select the correct objective in the Microscope Control tool (or the Microscope Components tool in ZEN lite), as well as the matching performed scaling in the status bar.

  1. The function Automatic Line Detection calculates the theoretical maximum of the reference line‘s both end points to the closest scale lines in the image. Thus, the distance will be calculated with sub-pixel accuracy.
  2. If you have defined a manual scaling for an objective and activate the checkbox Select Automatically, the software will use the measured scaling instead of the theoretic one. You will recognize this via the label Measured instead of Theoretic beside the pixel size.