By using this mode, you set the center plane of the z-stack. This mode is suitable if you know the thickness of your sample. In this case, it will be the fastest method to set up a z-stack.
- You are in the Z-Stack tool.
- Activate the Center mode by clicking Center on the top of the tool.
- In the Live mode, adjust the z-drive until you have exactly focused the center of the sample. The blue plane in the illustration shows the actual focus plane.
- Click Center under the settings section to set the actual focus position as center of the z-stack.
- Click Optimal to adjust the number of slices and the best interval according to the Nyquist criteria. Alternatively, you can set the desired interval and number of slices in the input fields manually.
- Depending on which option is selected in the Keep section, either the Interval or the Slices will be held constant.
- Click Start Experiment to start the experiment.
- You have successfully set up and performed a z-stack experiment using the Center mode.