If you want to perform the automatic calibration the holder must have been calibrated first by using the default calibration procedure. See Calibrating the S&F Holder. Performing auto-calibration the software tries to detect the exact positions of all three, small fiducial L-shaped markers on the holder automatically. The result of the detection will be presented afterwards. The automatic calibration is the easiest way to repeat the calibration process, e.g. if you have changed the sample on the holder and therefore removed the holder from the stage.

  1. You have inserted the holder to be calibrated on the stage (example SEM stage).
  1. Open the S&F Holder Calibration workbench.
  2. Check if the holder is still selected under Sample Holder.
  3. Activate Auto-Calibration starting with the first marker.
  4. The software will now start and try to detect all three positions of the small L-shaped markers.
  5. After the detection procedure you will see three preview images of the markers. On each image the red crosshair must be in the corner of the L-shaped marker.
  6. Click Finish calibration as soon as all three markers were detected correctly.
  7. If a marker was not detected correctly, click Edit Marker Positions to adjust the position manually.
  8. You will see a dialog which you can use to navigate to the detected positions and correct them afterwards. In our example Marker 3 was not detected correctly.
  9. Click Marker 3.
  10. The stage will move to the position which was not detected correctly.
  11. Move the stage by the joystick so that the small L-shaped marker is visible in the Live image and click Manual .
  12. Determine the position manually by left clicking in the corner of the L-shaped marker.
  13. Click on Finish Calibration. If you want to check if all three markers are detected correctly, you can navigate on the holder by clicking Marker 1-3 buttons.
  1. You have calibrated the holder successfully. You can now acquire images of your sample. Note the calibration process must be performed again, after the holder is transferred (shuttled) to the other system. Otherwise the same sample positions (ROIs/POIs) cannot be found automatically.