The Relative Image Brightness (Luminosity) is adjusted to 55% which means that the filter background indicated by the largest peak (gray value with the highest intensity in pixel counts) appears in the middle of the gray value range:
The Relative Threshold is set at 70% referring to the Relative Image Brightness (Luminosity) setting.
For information on setting the relative image brightness (Luminosity), see Camera Tool and Light Path Editing Tool.
For information on relative threshold, see Particle Segmentation Tool (Components).