Standardizing imaging conditions can greatly impact the complexity of a segmentation task. Standardizing imaging parameters can significantly simplify the task, making it easier for the algorithm to learn, and ultimately reducing the number of annotations required. To achieve this, consider the following guidelines when collecting images for your dataset to ensure optimal performance with the arivis AI toolkit:

• use the same illumination parameters for similar intensity histograms
• use the same magnification and binning for objects of similar pixel size
• keep the size of individual regions/objects to be segmented below 320*320 pixels