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Introduction The Spine Tracer module was introduced in arivis Pro 4.3 as a complement to the Neuron Tracer functionality introduced in arivis Vision4D 4.0. It is designed to identify and quantify neuronal spines.

Detecting spines with neurite/neuron tracer This article covers the Spine Tracer pipeline operation and how it can be used in conjunction with Neurite Tracer and Neuron Tracer operations.

Spine Tracer The Spine Tracer operation is used to detect dendritic spines on a neuron trace. The operation is based on the trace model and requires a trace object as input.

Spine Tracer The Spine Tracer operation is used to detect dendritic spines on a neuron trace. The operation is based on the trace model and requires a trace object as input.

Spine Tracer The Spine Tracer operation is used to detect dendritic spines on a neuron trace. The operation is based on the trace model and requires a trace object as input.

Overview Start by using either the Neuron Tracer or Neurite Tracer operations to create neurites. Use the Spine Tracer with existing neurites to detect and quantify spines.

Adding Spine Detection to a Pipeline As mentioned above, the first prerequisite for spine detection is having already detected neurites. We can use either the Neurite Tracer or Neuron Tracer for this task.

Spine Tracer parameters Parameter Description Method Sets the method for tracing spines.

Spine Tracer parameters Parameter Description Method Sets the method for tracing spines.

Spine Tracer parameters Parameter Description Method Sets the method for tracing spines.

arivis Pro 4.3 Released: 2025-03-11 New & Noteworthy AI-assisted Spine Tracing: automated spine detection using pre-trained Al models or other methods. Enhanced Cell Measurements: expanded measurement capabilities enabling richer feature sets.